sp1 sp3 inhibitor mithramycin Search Results


fusobacterium sp 1 1 41faa 1367 294782278 fusobacterium sp 3 1 27  (ATCC)
93
ATCC fusobacterium sp 1 1 41faa 1367 294782278 fusobacterium sp 3 1 27
Fusobacterium Sp 1 1 41faa 1367 294782278 Fusobacterium Sp 3 1 27, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against sp1 sp3  (Santa Cruz Biotechnology)
99
Santa Cruz Biotechnology antibodies against sp1 sp3
Antibodies Against Sp1 Sp3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal sp1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology rabbit polyclonal sp1
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Rabbit Polyclonal Sp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal sp1 - by Bioz Stars, 2026-10
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pcgn-sp1  (Paul N Gardner)
90
Paul N Gardner pcgn-sp1
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Pcgn Sp1, supplied by Paul N Gardner, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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for visual c++6.0  (InstallShield Software Corporation)
90
InstallShield Software Corporation for visual c++6.0
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
For Visual C++6.0, supplied by InstallShield Software Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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for visual c++6.0 - by Bioz Stars, 2026-10
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sp1 sp3 combined transfections  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sp1 sp3 combined transfections
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Sp1 Sp3 Combined Transfections, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cadm1 antibody igsf4 h00023705-m01 clone 2a5  (Abnova)
90
Abnova cadm1 antibody igsf4 h00023705-m01 clone 2a5
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Cadm1 Antibody Igsf4 H00023705 M01 Clone 2a5, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp1-tap  (CAMECA Inc)
90
CAMECA Inc sp1-tap
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Sp1 Tap, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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binding sites bκregion containing sp1/sp3  (Furuuchi Chemical Corp)
90
Furuuchi Chemical Corp binding sites bκregion containing sp1/sp3
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Binding Sites Bκregion Containing Sp1/Sp3, supplied by Furuuchi Chemical Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp1 sp3 sirna  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sp1 sp3 sirna
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Sp1 Sp3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp1–sp3 primers  (GeNOsys Inc)
90
GeNOsys Inc sp1–sp3 primers
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Sp1–Sp3 Primers, supplied by GeNOsys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp1 sp3 positive  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sp1 sp3 positive
Fig. 8. The <t>Sp1</t> reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.
Sp1 Sp3 Positive, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 8. The Sp1 reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.

Journal: Carcinogenesis

Article Title: 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expression.

doi: 10.1093/carcin/23.8.1297

Figure Lengend Snippet: Fig. 8. The Sp1 reporter activity in MCF-7 cells treated with DIM. MCF-7 cells were transiently transfected with Sp1-CAT reporter plasmid containing three Sp1 consensus binding elements. Cells were treated with DMSO or DIM (50 µM) for 24 h and CAT activities were determined as described above. Data shown were the mean SD from three independent experiments with duplicate samples.

Article Snippet: For antibody super-shift experiments, 1 μg of monoclonal mouse IgG anti-human-Sp1 or rabbit polyclonal Sp1 or Sp3 antibody (Santa Cruz Biotechnology, #sc-420X and #sc-644X) was added to the incubation mixture.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Binding Assay

Fig. 9. The stimulation of the binding activity of Sp1/Sp3 to their cognate DNA binding sequence with DIM treatment. Nuclear extracts were prepared from MCF-7 cells that were treated with DMSO or DIM (50 µM) for 48 h. The nuclear fractions (20 µg) were incubated with specific antibodies for Sp1 or Sp3 for 20 min and further incubated with radiolabeled Sp1 wild- type or mutated oligonucleotides for additional 15 min. The protein–DNA complexes were electrophoretically fractionated on non-denaturing 6% polyacrylamide gels. Gels were dried and exposed to X-ray film for radioautography. Arrows indicate the locations of Sp1 or Sp3 protein–DNA complexes.

Journal: Carcinogenesis

Article Title: 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expression.

doi: 10.1093/carcin/23.8.1297

Figure Lengend Snippet: Fig. 9. The stimulation of the binding activity of Sp1/Sp3 to their cognate DNA binding sequence with DIM treatment. Nuclear extracts were prepared from MCF-7 cells that were treated with DMSO or DIM (50 µM) for 48 h. The nuclear fractions (20 µg) were incubated with specific antibodies for Sp1 or Sp3 for 20 min and further incubated with radiolabeled Sp1 wild- type or mutated oligonucleotides for additional 15 min. The protein–DNA complexes were electrophoretically fractionated on non-denaturing 6% polyacrylamide gels. Gels were dried and exposed to X-ray film for radioautography. Arrows indicate the locations of Sp1 or Sp3 protein–DNA complexes.

Article Snippet: For antibody super-shift experiments, 1 μg of monoclonal mouse IgG anti-human-Sp1 or rabbit polyclonal Sp1 or Sp3 antibody (Santa Cruz Biotechnology, #sc-420X and #sc-644X) was added to the incubation mixture.

Techniques: Binding Assay, Activity Assay, Sequencing, Incubation

Fig. 10. Competitive gel-shift analysis of the binding of Sp1/Sp3 to their DNA element. Nuclear protein samples were prepared from MCF-7 cells treated with DMSO or DIM (50 µM) for 48 h. Nuclear extracts (20 µg) were pre-incubated with excess amounts of unlabeled wild-type or mutated oligonucleotides. Following a 20-min incubation, radiolabeled, wild-type Sp1 oligonucleotide probes were added and incubated for another 20 min. The protein–DNA complexes were resolved by electrophoresis and gels were dried and exposed to X-ray films. Arrows indicate the locations of Sp1 or Sp3 protein–DNA complexes.

Journal: Carcinogenesis

Article Title: 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expression.

doi: 10.1093/carcin/23.8.1297

Figure Lengend Snippet: Fig. 10. Competitive gel-shift analysis of the binding of Sp1/Sp3 to their DNA element. Nuclear protein samples were prepared from MCF-7 cells treated with DMSO or DIM (50 µM) for 48 h. Nuclear extracts (20 µg) were pre-incubated with excess amounts of unlabeled wild-type or mutated oligonucleotides. Following a 20-min incubation, radiolabeled, wild-type Sp1 oligonucleotide probes were added and incubated for another 20 min. The protein–DNA complexes were resolved by electrophoresis and gels were dried and exposed to X-ray films. Arrows indicate the locations of Sp1 or Sp3 protein–DNA complexes.

Article Snippet: For antibody super-shift experiments, 1 μg of monoclonal mouse IgG anti-human-Sp1 or rabbit polyclonal Sp1 or Sp3 antibody (Santa Cruz Biotechnology, #sc-420X and #sc-644X) was added to the incubation mixture.

Techniques: Gel Shift, Binding Assay, Incubation, Electrophoresis